Hydrophobically modified polyacrylamide block copolymers for fast, high-resolution DNA sequencing in microfluidic chips
نویسندگان
چکیده
منابع مشابه
Towards DNA Sequencing Chips
DNA sequencing is an important technology for the determination of the sequences of nucleotides that make up a given DNA fragment. In view of the limitations of current sequencing technology, it would be advantageous to have a DNA sequencing method that provides the sequences of long DNA fragments and is amenable to automation. Sequencing by Hybridization (SBH) is a challenging alternative to t...
متن کاملHigh resolution size analysis of ADP-ribose polymers using modified DNA sequencing gels.
Mammalian cells respond to DNA damage by producing polymers of ADP-ribose (ADPR) on certain DNA-binding proteins (1). Synthesis of variably-sized ADPR polymers correlates with modified protein function (2). Here we present a high resolution electrophoresis technique for separating and quantifying picomole quantities of polymers up to 60 residues long. This technique allows one to study the role...
متن کاملMicrofluidic Chips for Liposome Production
In recent years, the development of nanoand micro-particles has attracted considerable 11 interest from researchers and enterprises, because of the potential utility of such particles as drug 12 delivery vehicles. Amongst the different techniques employed for the production of nanoparticles, 13 microfluidic-based methods have proven to be the most effective for controlling particle size and 14 ...
متن کاملMicrofluidic Chips for Semen Analysis
The gold standard of semen analysis is still an manual method, which is time-consuming, labour intensive and needs thorough quality control. Microfluidics can also offer advantages for this application. Therefore a first step in the development of a microfluidic chip has been made, which enables the man the semen analysis at home. In this article recent efforts to determine the concentration an...
متن کاملComparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2008
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.200800353